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U-to-C RNA editing by synthetic PPR-DYW proteins in bacteria and human culture cells.

Ichinose M, Kawabata M, Akaiwa Y, Shimajiri Y, Nakamura I, Tamai T, Nakamura T, Yagi Y, Gutmann B
Commun Biol. 2022 Sep 15;5(1):968. doi: 10.1038/s42003-022-03927-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190955pET-PLS-KP1_rpoA-UTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli
190956pET-PLS-KP2_rpoA-UTest the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli
190957pET-PLS-KP2-A66S_rpoA-UTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli
190958pET-PLS-KP2-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli
190959pET-PLS-KP2-E67A_rpoA-UTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli
190960pET-PLS-KP3_rpoA-UTest the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli
190961pET-PLS-KP3-A66S_rpoA-UTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli
190962pET-PLS-KP3-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli
190963pET-PLS-KP3-E67A_rpoA-UTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli
190964pET-PLS-KP4_rpoA-UTest the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli
190965pET-PLS-KP5_rpoA-UTest the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli
190966pET-PLS-KP6_rpoA-CTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli
190967pET-PLS-KP6_rpoA-UTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli
190968pET-PLS-KP6-A66S_rpoA-CTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli
190969pET-PLS-KP6-A66S_rpoA-UTest the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli
190970pET-PLS-KP6-C93A-C96A_rpoA-CTest the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli
190971pET-PLS-KP6-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli
190972pET-PLS-KP6-E67A_rpoA-CTest the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli
190973pET-PLS-KP6-E67A_rpoA-UTest the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli
190974pET-PLS-KP7_rpoA-UTest the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli
190975PM18033-PLS-KP1_rpoA-UTest the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells
190976PM18033-PLS-KP2_rpoA-UTest the U-to-C editing activity of KP2 on AtrpoA editing site, in human cells
190977PM18033-PLS-KP2-A66S_rpoA-UTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in human cells
190978PM18033-PLS-KP2-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in human cells
190979PM18033-PLS-KP2-E67A_rpoA-UTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in human cells
190980PM18033-PLS-KP3_rpoA-UTest the U-to-C editing activity of KP3 on AtrpoA editing site, in human cells
190981PM18033-PLS-KP3-A66S_rpoA-UTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in human cells
190982PM18033-PLS-KP3-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in human cells
190983PM18033-PLS-KP3-E67A_rpoA-UTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in human cells
190984PM18033-PLS-KP4_rpoA-UTest the U-to-C editing activity of KP4 on AtrpoA editing site, in human cells
190985PM18033-PLS-KP5_rpoA-UTest the U-to-C editing activity of KP5 on AtrpoA editing site, in human cells
190986PM18033-PLS-KP6_rpoA-CTest the C-to-U editing activity of KP6 on AtrpoA editing site, in human cells
190987PM18033-PLS-KP6_rpoA-UTest the U-to-C editing activity of KP6 on AtrpoA editing site, in human cells
190988PM18033-PLS-KP6-A66S_rpoA-CTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in human cells
190989PM18033-PLS-KP6-A66S_rpoA-UTest the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in human cells
190990PM18033-PLS-KP6-C93A-C96A_rpoA-CTest the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells
190991PM18033-PLS-KP6-C93A-C96A_rpoA-UTest the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells
190992PM18033-PLS-KP6-E67A_rpoA-CTest the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in human cells
190993PM18033-PLS-KP6-E67A_rpoA-UTest the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in human cells
190994PM18033-PLS-KP7_rpoA-UTest the U-to-C editing activity of KP7 on AtrpoA editing site, in human cells

Antibodies from Article