Semi-automated workflows to quantify AAV transduction in various brain areas and predict gene editing outcome for neurological disorders.
Duarte F, Ramosaj M, Hasanovic E, Regio S, Sipion M, Rey M, Deglon N
Mol Ther Methods Clin Dev. 2023 Mar 27;29:254-270. doi: 10.1016/j.omtm.2023.03.013. eCollection 2023 Jun 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190898 | pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2 | Entry vector for multiple sgRNA cloning |
| 190899 | pAAV2ss-U6-sgGFP | AAV vector expressing sgGFP |
| 190900 | pAAV2ss-U6-sgHTT1-7sk-sgCas9 | AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs |
| 190901 | pAAVss-U6-sgHTT51-7sk-Cas9 | AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA |
| 190902 | pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 | AAV vector expressing thew GFP reporter gene and human sgHTT |
| 190903 | pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 | AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9 |