Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on beta-catenin.
Zhu Y, Hart GW
Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190821 | pET24a-ncOGT-FL | For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli. |
| 190822 | pET24a-ncOGT-TPR | For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli. |
| 190824 | pEGFP-β-catenin_S23A,T40A,T41A,T112A | For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus. |
| 190825 | pSilencer2.1-U6-T1 | For mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter. |
| 190826 | pSilencer2.1-U6-AP3 | For mammalian expression of AP3, the RNA aptamer of GFP. Expression is driven by a U6 promoter. |
| 190827 | pSilencer2.1-U6-17-6 | For mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter. |
| 190828 | pSilencer2.1-U6-T1-U6-AP3 | For mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter. |
| 190829 | pSilencer2.1-U6-NL1F30 | For mammalian expression of NL1F30, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190830 | pSilencer2.1-U6-NL1F35 | For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190831 | pSilencer2.1-U6-NL1F50 | For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190832 | pSilencer2.1-U6-3JB1F | For mammalian expression of 3JB1F, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190833 | pSilencer2.1-U6-3JB1F+2 | For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190834 | pSilencer2.1-U6-3JB1F+4 | For mammalian expression of 3JB1F+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190835 | pSilencer2.1-U6-3JB1F+6 | For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190836 | pSilencer2.1-U6-3JB1F+8 | For mammalian expression of 3JB1F+8, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190837 | pSilencer2.1-U6-3JB1F+10 | For mammalian expression of 3JB1F+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190838 | pSilencer2.1-U6-3JB1F+12 | For mammalian expression of 3JB1F+12, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190839 | pSilencer2.1-U6-3JB1R | For mammalian expression of 3JB1R, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190840 | pSilencer2.1-U6-3JB1R+2 | For mammalian expression of 3JB1R+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190841 | pSilencer2.1-U6-3JB1R+4 | For mammalian expression of 3JB1R+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190842 | pSilencer2.1-U6-3JB1R+6 | For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190843 | pSilencer2.1-U6-3JB1R+8 | For mammalian expression of 3JB1R+8, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190844 | pSilencer2.1-U6-3JB1R+10 | For mammalian expression of 3JB1R+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190845 | pSilencer2.1-U6-3JB1R+12 | For mammalian expression of 3JB1R+12, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190846 | pSilencer2.1-U6-4JC1FF | For mammalian expression of 4JC1FF, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190847 | pSilencer2.1-U6-4JC1RR | For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190848 | pSilencer2.1-U6-bc339 | For mammalian expression of bc339, the RNA aptamer of β-catenin. Expression is driven by a U6 promoter. |
| 190849 | pSilencer2.1-U6-T1-U6-bc339 | For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter. |
| 190850 | pSilencer2.1-U6-NL8F50 | For mammalian expression of NL8F50, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190851 | pSilencer2.1-U6-NL8F70 | For mammalian expression of NL8F70, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190852 | pSilencer2.1-U6-NL8F100 | For mammalian expression of NL8F100, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker. |
| 190853 | pSilencer2.1-U6-3JB8F+4 | For mammalian expression of 3JB8F+4, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190854 | pSilencer2.1-U6-3JB8F+12 | For mammalian expression of 3JB8F+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190855 | pSilencer2.1-U6-3JB8R+12 | For mammalian expression of 3JB8R+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker. |
| 190856 | pSilencer2.1-U6-LRS1F50C | For mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements. |
| 190858 | pcDNA3.1-OGT_opt | For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells. |