Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection.
Sloas DC, Tran JC, Marzilli AM, Ngo JT
Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190801 | LV UAS-DsRed-Express2-PGK-Puro | LV construct encoding UAS-DsRed-Express2 reporter gene. |
| 194929 | LV anti-FITC-LaG16-NRR-T2A-BFP | LV construct encoding NRR-based SynNotch receptor |
| 194930 | LV anti-FITC-LaG16-sNRR-T2A-BFP | LV construct encoding sNRR-based SynNotch receptor |
| 194931 | LV anti-FITC-LaG16-Y186F-T2A-BFP | LV construct encoding Y186F mutant sNRR-based SynNotch receptor |
| 194932 | LV anti-FITC-LaG16-Y186A-T2A-BFP | LV construct encoding Y186A mutant sNRR-based SynNotch receptor |
| 194933 | LV anti-FITC-LaG16-Y192A-T2A-BFP | LV construct encoding Y192A mutant sNRR-based SynNotch receptor |
| 194934 | LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP | LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor |
| 194935 | LV anti-FITC-LaG16-R103A-T2A-BFP | LV construct encoding R103A mutant sNRR-based SynNotch receptor |
| 194936 | LV TRE-GFP-TMD-DLL1 | LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette. |
| 194937 | LV TRE-GFP-TMD-DLL1-Nbox-mutant | LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette. |
| 195854 | pBAD-6xHis-AviTag-ALFAtag-eGFP | Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence. |