Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803.
Cengic I, Canadas IC, Minton NP, Hudson EP
ACS Synth Biol. 2022 Aug 15. doi: 10.1021/acssynbio.2c00375.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190790 | pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA | [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system. |
| 190793 | pMD19-PBBa_J23117-sgRNA-Spr | Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites. |
| 190794 | pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr | Template vector to amplify pieces for creating multiplex sgRNA-arrays. |