Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy
Foust DJ, Piston DW
bioRxiv 2021.11.03.467169
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190745 | pCD86-mEGFP | Expression of mEGFP-tagged CD86 in mammalian cells. |
| 190746 | pCD86-mEYFP(Q69K) | Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells. |
| 190747 | pCD86-mCherry2 | Expression mCherry2-tagged CD86 in mammalian cells. |
| 190748 | pmCherry2-CD86-mEGFP | Expression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells. |
| 190749 | pmCherry2-CD86-mEYFP(Q69K) | Expression of CD86 double tagged with mCherry2 (N-term) and mEYFP(Q69K) (C-term) in mammalian cells. |
| 190750 | pCD86-mEYFP(Q69K)-mEGFP | Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells. |
| 190751 | pmCherry2-CD86-mEYFP(Q69K)-mEGFP | Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells. |
| 190752 | pmEGFP-DRD2 | Expression of DRD2 with mEGFP tag on N-terminus. |
| 190753 | pmEGFP-ADRA2A | Expression of ADRA2A with mEGFP tag on N-terminus. |
| 190755 | pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K) | Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags. |