Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces.

Ameruoso A, Villegas Kcam MC, Cohen KP, Chappell J
Nucleic Acids Res. 2022 Jul 22;50(13):7751-7760. doi: 10.1093/nar/gkac556. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
190814	pJEC726	CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
190815	pJEC819	CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.