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Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor.

Cao X, Shami Shah A, Sanford EJ, Smolka MB, Baskin JM
ACS Chem Biol. 2022 Aug 11. doi: 10.1021/acschembio.2c00527. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190736pCDNA3-EMTB-TurboID-V5Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
190740pCDNA3-DCX-TurboID-V5Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
190741pCDH-EMTB-TurboID-V5-puroA lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
190742pCDH-DCX-TurboID-V5-puroA lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Antibodies from Article