Maja Jagodic Lab: Implication of DNA methylation changes at chromosome 1q21.1 in the brain pathology of Primary Progressive Multiple Sclerosis

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
190728	P3-dCas9-Tet1-GFP-Puro	Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
190729	P3-Lenti-dCas9-Tet1-GFP	Lentiviral construct to express spdCas9-Tet1-GFP.