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Addgene

De novo designed peptides for cellular delivery and subcellular localisation.

Rhys GG, Cross JA, Dawson WM, Thompson HF, Shanmugaratnam S, Savery NJ, Dodding MP, Hocker B, Woolfson DN
Nat Chem Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 10.1038/s41589-022-01076-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190706pVRC-N-Venus-V150A-apCC-DiEncodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.
190707pVRC-N-Venus-V150A-CC-DiEncodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.
190708pVRC-N-Venus-V150AControl plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.
190709pBAD-apCC-Di-C-VenusEncodes the antiparallel coiled-coil apCC-Di followed by C-Venus.
190710pBAD-CC-Di-C-VenusEncodes the parallel coiled-coil CC-Di followed by C-Venus.
190711pBAD-C-Venus-apCC-DiEncodes C-Venus followed by the antiparallel coiled-coil apCC-Di.
190712pBAD-C-Venus-CC-DiEncodes C-Venus followed by the parallel coiled-coil CC-Di.
190713pBAD-C-VenusControl plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.

Antibodies from Article