Overexpression and ELISA-based detection of asprosin in cultured cells and mice.
Mishra I, Chopra AR
STAR Protoc. 2022 Dec 16;3(4):101762. doi: 10.1016/j.xpro.2022.101762. Epub 2022 Oct 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190683 | pcDNA3.1-IL2sp-6His-Asprosin plasmid | Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells. |