Simplified cloning and isolation of peptides from "sandwiched" SUMO-peptide-intein fusion proteins.
Lamer T, Vederas JC
BMC Biotechnol. 2023 Apr 5;23(1):11. doi: 10.1186/s12896-023-00779-5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
190676 | pSPIH6 | E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein) |