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Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus.

Lutrat C, Olmo RP, Baldet T, Bouyer J, Marois E
Commun Biol. 2022 Mar 7;5(1):210. doi: 10.1038/s42003-022-03165-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
173496pPiggyBacATCC LacZ GCTT loxAttPpiggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies
173505ppBAalbNixE1E2 OpIE2-GFPtransgenesis vector for Aedes albopictus ectopic expression of the Nix gene
173665ppBAalbNixE1E2E3E4 PUb-DsRedtransgenesis vector for Aedes albopictus ectopic expression of the Nix gene
173666ppBAalbNixE1E3E4 PUb-YFPtransgenesis vector for Aedes albopictus ectopic expression of the Nix gene
173667ppBAalbNixE1E2 PUb-GFPtransgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Antibodies from Article