A truncated reverse transcriptase enhances prime editing by split AAV vectors.
Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan SR, Thomsen MK, Mikkelsen JG, Bak RO
Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 10.1016/j.ymthe.2022.07.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190335 | PE_CO | Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells |
| 190336 | PE_CO-Mini-N | Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion |
| 190337 | PE_CO-Mini-C | Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion |
| 190338 | LV-PE_CO-Mini | Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase) |