Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding.
Sang D, Shu T, Pantoja CF, Ibanez de Opakua A, Zweckstetter M, Holt LJ
Mol Cell. 2022 Oct 6;82(19):3693-3711.e10. doi: 10.1016/j.molcel.2022.08.016. Epub 2022 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190248 | pRS306-PTDH3-SUMO10-6SIM | Expression SUMO10-SIM6 in yeast cells |
| 190249 | pRS306-PTDH3-Dimer-1SUMO | Expression dimer-1SUMO component in yeast cells |
| 190250 | pRS306-PTDH3-SUMO7-6SIM | Expression SUMO7-SIM6 in yeast cells |
| 190251 | pRS304-PTDH3-HEXAMER | Expression hexamer component in yeast |
| 190252 | pAV103-PRNR2-mCherry-Elk1DM-1SIM | Expression mCherry-Elk1DM in yeast cells |
| 190253 | pAV105-PPAB1-GFP-Erk1R84S-1SIM | Expression Erk1-GFP-1SIM in yeast cells |