Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms.
Soto JS, Jami-Alahmadi Y, Chacon J, Moye SL, Diaz-Castro B, Wohlschlegel JA, Khakh BS
Nature. 2023 Apr;616(7958):764-773. doi: 10.1038/s41586-023-05927-7. Epub 2023 Apr 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176740 | pZac2.1 gfaABC1D-BioID2-BioID2-HA | Expresses the promiscuous small biotin ligase, BioID2, in astrocytes |
| 176741 | pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA | Expresses membrane bound biotin ligase, BioID2, in astrocytes |
| 176742 | pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA | Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes |
| 176743 | pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA | Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes |
| 176744 | pZac2.1 gfaABC1D-Ezr-BioID2-HA | Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes |
| 176745 | pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA | Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes |
| 176746 | pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA | Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes |
| 176747 | PZac2.1 gfaABC1D-Aqp4-eGFP | Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes |
| 176853 | PZac2.1 hSyn1-eGFP-SAPAP3 | Expresses SAPAP3 protein fused with eGFP at the N-terminus in neurons |
| 176854 | PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA | Expresses membrane bound biotin ligase, BioID2, in neurons |
| 176855 | PZac2.1 hSyn1-BioID2-BioID2-HA | Expresses the promiscuous small biotin ligase, BioID2, in neurons |
| 176856 | pZac2.1 gfaABC1D-Ezr-eGFP | Expresses Ezrin fused with eGFP at the C-terminus in astrocytes |
| 176857 | PZac2.1 hSyn1-HA-BioID2-SAPAP3 | Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons |
| 176858 | PZac2.1 gfaABC1D-HA-BioID2-SAPAP3 | Expresses SAPAP3 protein fused with BioID2 at the N-terminus in astrocytes |
| 176859 | PZac2.1 gfaABC1D-eGFP-SAPAP3 | Expresses SAPAP3 protein fused with eGFP at the N-terminus in astrocytes |
| 176860 | PZac2.1 gfaABC1D-Cx43-GFP | Expresses Cx43 fused with GFP at the C-terminus in astrocytes |
| 176861 | pZac2.1 gfaABC1D-eGFP | Expresses cytosolic eGFP in astrocytes |
| 176862 | pZac2.1 gfaABC1D-Glt1-eGFP | Expresses Glt-1 fused with GFP at the C-terminus in astrocytes |
| 177333 | PZac2.1 hSyn1-Lck-GFP | Expresses GFP on the plasma membrane of neurons via an Lck localization sequence |
| 190199 | pZac2.1 gfaABC1D-LifeAct-GFP | Expresses the small actin binding peptide, LifeAct, with a GFP reporter |
| 190200 | pZac2.1 gfaABC1D-3xHA-SAPAP3 | Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes |