Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean.
Bai M, Yuan J, Kuang H, Gong P, Li S, Zhang Z, Liu B, Sun J, Yang M, Yang L, Wang D, Song S, Guan Y
Plant Biotechnol J. 2020 Mar;18(3):721-731. doi: 10.1111/pbi.13239. Epub 2019 Sep 9.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
190197 | pGES201 | For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection |