A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9.
Aguilar RR, Shen ZJ, Tyler JK
Methods Protoc. 2022 Oct 4;5(5):79. doi: 10.3390/mps5050079.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190175 | pCASB | Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence |