Opto-katanin, an optogenetic tool for localized, microtubule disassembly.
Meiring JCM, Grigoriev I, Nijenhuis W, Kapitein LC, Akhmanova A
Curr Biol. 2022 Nov 7;32(21):4660-4674.e6. doi: 10.1016/j.cub.2022.09.010. Epub 2022 Sep 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190165 | EB3N-VVDfast-mCl3-iLID | Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells. |
| 190166 | EB3N-VVDfast-Halo-iLID | Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells. |
| 190167 | iLID-mCl3-MAP7N | Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells. |
| 190168 | SspBmicro-mCh-p60 | Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells. |
| 190169 | pSIN-TRE-EB3N-VVDfast-mCl3-iLID | Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60. |
| 190170 | pSIN-TRE-SspB-mCh-p60 | Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells. |
| 190171 | Halo-Rab6A | Expresses HaloTag-Rab6A in mammalian cells. |