CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.
Lam M, Takeo K, Almeida RG, Cooper MH, Wu K, Iyer M, Kantarci H, Zuchero JB
Nat Commun. 2022 Sep 23;13(1):5583. doi: 10.1038/s41467-022-33200-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190151 | pCMV-Vamp2-pHluorin | Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis |
| 190152 | pCMV-Vamp3-pHluorin | Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis |
| 190153 | AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax | Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector |
| 190154 | AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax | Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector |
| 190155 | AAV_pMBP-EGFP-caax | Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector |