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AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii.

Brychcy M, Kokodynski A, Lloyd D, Godoy VG
ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. (Link opens in a new window) PubMed (Link opens in a new window) Article


A subset of these plasmids (#190114 to #190132) are available to purchase together in the AspFlex Kit (Kit #1000000217).

Plasmids from Article

ID Plasmid Purpose
190114pMB1-ALevel 1 MoClo plasmid for A. baumannii
190115pMB1-BLevel 1 MoClo plasmid for A. baumannii
190116pMB1-CLevel 1 MoClo plasmid for A. baumannii
190117pMB1-DLevel 1 MoClo plasmid for A. baumannii
190118pMB1-D1Level 1 MoClo plasmid for A. baumannii
190119pMB1-ELevel 1 MoClo plasmid for A. baumannii
190120pMB2aLevel 2 MoClo plasmid for A. baumannii
190121pMB2bLevel 2 MoClo plasmid for A. baumannii
190122pMB2-ALevel 2 MoClo plasmid for A. baumannii
190123pMB2-BLevel 2 MoClo plasmid for A. baumannii
190124pMB2-CLevel 2 MoClo plasmid for A. baumannii
190125pMB2-DLevel 2 MoClo plasmid for A. baumannii
190126pMB3-ALevel 2 MoClo plasmid for A. baumannii
190127pMB3-BLevel 2 MoClo plasmid for A. baumannii
190128pBP_sgRNALevel 0 MoClo plasmid for Golden Gate cloning of sgRNAs
190129pMB1-A-ptet-dCas9Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi
190130pMB1-A-ptet-FLAG-dCas9Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi
190131pBP_dCas9Level 0 MoClo plasmid containing dCas9
190132pMB1-A-pBAD-dCas9Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi
204979pMB1-A-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204980pMB1-B-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204981pMB1-C-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204982pMB1-D-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204983pMB1-D1-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204984pMB1-E-TelRLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204985pMB2a-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204986pMB2b-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204987pMB2-A-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204988pMB2-B-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204989pMB2-C-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204990pMB2-D-TelRLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204991pMB3-A-TelRLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
204992pMB3-B-TelRLevel 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Antibodies from Article