Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
Grunewald J, Miller BR, Szalay RN, Cabeceiras PK, Woodilla CJ, Holtz EJB, Petri K, Joung JK
Nat Biotechnol. 2023 Mar;41(3):337-343. doi: 10.1038/s41587-022-01473-1. Epub 2022 Sep 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190104 | SpCas9 nickase for Split-PE (pJUL2497) | Expresses nickase SpCas9(H840A) for Split-PE use |
| 190105 | MMLV-RT for Split-PE (pEH45) | Expresses MMLV-RT (full-length, from PE2) for Split-PE use |
| 190106 | MMLV-RT(dRH) for Split-PE (pEH50) | Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use |
| 190107 | HERV-Kcon-RT for Split-PE (pJUL3120) | Expresses HERV-Kcon RT (human codon optimization) |
| 190108 | Marathon-RT for Split-PE (pEH59) | Expresses Marathon-RT (human codon optimization) |
| 190109 | Marathon-RT tetramutant for Split-PE (pEH101) | Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R) |
| 190110 | Marathon-RT pentamutant for Split-PE (pEH107) | Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R) |
| 190111 | AAV1 for Split-PE, SpCas9 nickase (PKC1001) | AAV1 for expression of nickase SpCas9(H840A) for Split-PE use |
| 190112 | AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002) | AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP |