Protocol for production and purification of SARS-CoV-2 3CL(pro).
Mazzei L, Greene-Cramer R, Bafna K, Jovanovic A, De Falco A, Acton TB, Royer CA, Ciurli S, Montelione GT
STAR Protoc. 2023 May 5;4(2):102326. doi: 10.1016/j.xpro.2023.102326.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190062 | pGTM_COV2_NSP5_004_SUMO | Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease |
| 190063 | pGTM_YR375_SUMO_Protease_001 | Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion |
| 192349 | pGTM_COV2_NSP5_C145A_001_SUMO | Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form |