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PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as Signal Integrator.

Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 10.1021/acssynbio.2c00138. Epub 2022 Aug 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190051pBeast-pAhpC-RBS- sfGFPGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter
190052pBeast-pOxyS-RBS- sfGFPGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter
190053pBeast-pKatG-RBS- sfGFPGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter
190054pBeast-pYjjZ-RBS- sfGFPGFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter
190057pBeast-pT7-soxAExpress the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7
190058pBeast-pT7-codAExpress the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7
190059pBeast-pT7-loxExpress the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7
190060pBeast-pAhpC-RBS- LacZLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Antibodies from Article