Spatial engineering of E. coli with addressable phase-separated RNAs.
Guo H, Ryan JC, Song X, Mallet A, Zhang M, Pabst V, Decrulle AL, Ejsmont P, Wintermute EH, Lindner AB
Cell. 2022 Sep 29;185(20):3823-3837.e23. doi: 10.1016/j.cell.2022.09.016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191121 | prCAG-MS2 | Transcription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoter |
| 191122 | prCAG-BoxB | Transcription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoter |
| 191123 | pMS2 | Transcription of 12xMS2 repeats from a tetracycline-inducible promoter |
| 191126 | pPN-mKate (PT7LacO-PN-mKate) | Expression of protein N tagged with mKate from a T7-lac inducible promoter |
| 191130 | pVioCD53 | Expression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoter |
| 191132 | placZα-tdMCP | Expression of tdMCP from a lacZα inducible promoter |
| 191400 | prCAG-RBS"m1"-sfGFP | Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1) |