Measuring Transcription Dynamics of Individual Genes Inside Living Cells.
Brouwer I, de Kort MAC, Lenstra TL
Methods Mol Biol. 2024;2694:235-265. doi: 10.1007/978-1-0716-3377-9_12.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
189940 | SIVURA MCP-GFPenvy | Single-integration vector for integration of MCP-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae |
190003 | MCP-EGFP-PiggyBac | Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP. |