Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors.
Davis JR, Wang X, Witte IP, Huang TP, Levy JM, Raguram A, Banskota S, Seidah NG, Musunuru K, Liu DR
Nat Biomed Eng. 2022 Jul 28. pii: 10.1038/s41551-022-00911-4. doi: 10.1038/s41551-022-00911-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189922 | AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI | AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation |
| 189923 | AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI | AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation |
| 189924 | AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI | AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation |
| 189925 | AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI | AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation |
| 189927 | CMV-SauriABE8e | Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection. |
| 189928 | CMV-Nme2ABE8e | Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection. |
| 189929 | CMV-CjABE8e | Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection. |