Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes.
Hindul NL, Jhita A, Oprea DG, Hussain TA, Gonchar O, Campillo MAM, O'Regan L, Kanemaki MT, Fry AM, Hirota K, Tanaka K
Biol Open. 2022 Feb 15;11(2). pii: 274087. doi: 10.1242/bio.059056. Epub 2022 Feb 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190040 | pInt-ERCreER | This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833). |