mScarlet3: a brilliant and fast-maturing red fluorescent protein.

Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, Giepmans BNG, Aumonier S, Dupuy J, Royant A
Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
189752	pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3	Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
189753	pmScarlet3_C1	C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein
189754	pDx_mScarlet3	Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein
189755	pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3	Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
189756	pmScarlet-I3_C1	C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein
189757	pDx_mScarlet-I3	Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein
189758	pDx_mRuby3-SYFP2	Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis
189759	pDx_mCherry-SYFP2	Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis
189760	pDx_FusionRed-SYFP2	Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis
189761	pDx_mScarlet-I-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis
189762	pDx_mScarlet-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis
189763	pDx_mScarlet-I3-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis
189764	pDx_mScarlet3-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis
189765	pDx_mScarlet-I3-NCwt-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis
189766	pDx_mScarlet3-NCwt-SYFP2	Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis
189767	pLifeAct-mScarlet3_N1	Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3
189768	pmScarlet3-alphaTubulin_C1	Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin
189769	pmScarlet3-Peroxi_C1	Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3
189770	pEB3-mScarlet3_N1	Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3
189771	pLCK-mScarlet3_C1	Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3
189772	pER-mScarlet3_N1	Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3
189773	pmScarlet3-Giantin_C1	Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion
189774	p4xmts-mScarlet3_N1	Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3
189775	p3xnls-mScarlet3_N1	Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3
189776	pmScarlet3-LaminB_C1	Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion
189777	pCyterm-mScarlet-I3_N1	Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay
189778	pCyterm-mScarlet3_N1	Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay
189779	pCyterm-mScarlet-I3-NCwt_N1	Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay
224436	pCS2FA-H2B-mScarlet3	Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3