Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii.
Lehmayer L, Bernauer L, Emmerstorfer-Augustin A
iScience. 2022 Aug 6;25(9):104888. doi: 10.1016/j.isci.2022.104888. eCollection 2022 Sep 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189724 | pPpT4- PGK1prom-TIR1-FLAG | Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454) |
| 189725 | pPpT4- HTA1prom-TIR1-FLAG | Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001) |
| 189726 | pPpT4- TEF2prom-TIR1-FLAG | Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002) |
| 189727 | pPpT4- TEF2prom-TIR1F74G-FLAG | Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219) |
| 189728 | pPpT4- HTA1prom-TIR1 F74G -FLAG | Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220) |