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Molecular basis for the enzymatic macrocyclization of multiply backbone N-methylated peptides

Emmanuel Matabaro, Haigang Song, Fabio Gherlone, Lukas Sonderegger, Andrew Giltrap, Sam Liver, Alvar Gossert, Markus Künzler, James H Naismith
bioRxiv 2022.07.21.500988 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
189670His8OphMA-21mer.His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.
189671His8OphMADeltaC6-18mer.His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides
189672HisSumoOphPHisSUMO tagged OphP to express OphP in pichia pastorias.

Antibodies from Article