Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor.
Xiong LL, Garrett MA, Buss MT, Kornfield JA, Shapiro MG
ACS Synth Biol. 2022 Jul 15;11(7):2518-2522. doi: 10.1021/acssynbio.2c00093. Epub 2022 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195553 | pCIwt-PRM-Wasabi | Always-on expression of mWasabi reporter with transcription activation by wildtype CI |
| 195554 | pTcI38-PRM-wasabi | Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38 |
| 195555 | pTcI39-PRM-wasabi | Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39 |
| 195557 | pTcI39-switch | Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1 |
| 195560 | pBaseline-PRM-wasabi | Always-off, unactivated expression of mWasabi reporter |