Optimized metrics for orthogonal combinatorial CRISPR screens.
Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Susser S, Vera-Guapi A, Muller K, Matthess Y, Quandt E, Schaubeck S, Beisel CL, Kaulich M
Sci Rep. 2023 May 6;13(1):7405. doi: 10.1038/s41598-023-34597-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189632 | pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 | Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries |
| 189633 | pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro | Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries |
| 189634 | pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro | Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries |
| 189635 | pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro | Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries |
| 189636 | pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro | Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries |