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Addgene

Haian Fu Lab: TREAT-AD plasmids 2022

Unpublished

Plasmids from Article

ID Plasmid Purpose
189538pSCM167-Flag-hSFRP1Protein expression of SFRP1 in mammalian cells.
189539pDEST26-his-hSFRP1Protein expression of SFRP1 in mammalian cells.
189540pcDNA3.2-V5-DEST-hSFRP1-hisProtein expression of SFRP1 in mammalian cells.
189541pSCM167.2-Venus-VF-hSFRP1Protein expression of SFRP1 in mammalian cells.
189542pcDNA3.2-V5-DEST-hSFRP1-VFProtein expression of SFRP1 in mammalian cells.
189543pcDNA3.2-V5-DEST-hSFRP1-GSTProtein expression of SFRP1 in mammalian cells.
189544pcDNA3.2-V5-DEST-hSFRP1(aa53-166)Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.
189545pDEST27-GST-hSFRP1(aa53-169)Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
189546pSCM167-Venus-VF-hSFRP1(aa53-169)Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
189547pSCM167-Flag-hSFRP1(aa53-169)Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
189548pDEST27-GST-hSFRP1(aa181-314)Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
189549pcDNA3.2-V5-DEST-hSFRP1(aa181-314)-hisProtein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
189550pSCM167-Venus-VF-hSFRP1(aa181-314)Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
189551pSCM167-Flag-hSFRP1(aa181-314)Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
189552pSCM167.2-Venus-VF-hADAM10Protein expression ADAM10 in mammalian cells.
189553pDEST27-GST-hADAM10Protein expression ADAM10 in mammalian cells.
189554pSCM167.2-Venus-VF-hADAM10(aa1-213)Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
189555pSCM167-Venus-VF-hADAM10(aa220-459)Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459
189556pSCM167.2-Venus-VF-hADAM10(aa455-550)Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550
189557pSCM167.2-Venus-VF-hADAM10(aa551-654)Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654
189558pSCM167.2-Venus-VF-hADAM10(aa1-654)Protein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654
189559pSCM167-Venus-VF-hADAM10(aa1-459)Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459
189560pSCM167-Venus-VF-hADAM10(aa220-654)Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654
189561pSCM167-Venus-VF-hADAM10(aa455-654)Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654
189562pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GSTProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

Antibodies from Article