Activity-based directed evolution of a membrane editor in mammalian cells.

Tei R, Bagde SR, Fromme JC, Baskin JM
Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
188994	pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX	A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN
188993	pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX	A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN
188992	pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX	A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN
188991	pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX	A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN
188990	pCDH-p18-CIBN	A lentiviral plasmid encoding lysosome-tagged CIBN
188989	pCDH-CIBN-CAAX	A lentiviral plasmid encoding plasma membrane-tagged CIBN
188988	pCDH-CRY2-mCh-superPLDx30	A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)
188986	pCDH-CRY2-mCh-superPLDx10	A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)