Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation.
Chen Y, Jin S, Zhang M, Hu Y, Wu KL, Chung A, Wang S, Tian Z, Wang Y, Wolynes PG, Xiao H
Nat Commun. 2022 Sep 16;13(1):5434. doi: 10.1038/s41467-022-33111-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188983 | pEvol-NnSULT1C1-cysDNCQ | biosynthesize sulfotyrosine in E. coli. |
| 188984 | pET22b-T5-chi28TAG | Express chimadanin with TAG at 28 position |
| 188985 | pET22b-T5-chi31TAG | Express chimadanin with TAG at 31 position |
| 188996 | pET22b-T5-chi28TAG31TAG | Express chimadanin with TAG at 28 and 31 position |
| 188997 | pET22b-T5-mad32TAG | Express madanin-1 with TAG at 32 position |
| 188998 | pET22b-T5-mad | Express wildtype madanin-1 |
| 188999 | pET22b-T5-mad32TAG35TAG | Express madanin-1 with TAG at 32 and 35 position |