CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids.
Kim SK, Kim H, Woo SG, Kim TH, Rha E, Kwon KK, Lee H, Lee SG, Lee DH
Nucleic Acids Res. 2022 Dec 9;50(22):13155-13171. doi: 10.1093/nar/gkac1104.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188963 | pSLiP-G1 | L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown |
| 188964 | pSLiP-G2 | L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown |
| 188965 | pSLiP-G1G2 | L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown |
| 188966 | pSLiP-G2-RacE | L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown |
| 188967 | pT-GFP-rG1 | IPTG inducible GFP with sgRNA |
| 188968 | pT-GFP-rG2 | IPTG inducible GFP with sgRNA |
| 188969 | pA-RFP-rG2 | IPTG inducible mCherry with sgRNA |