High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome.
Schwartz ML, Davis MW, Rich MS, Jorgensen EM
PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 10.1371/journal.pgen.1009755. eCollection 2021 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188881 | pMLS538 | Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2) |
| 188882 | pMLS544 | Integrating Pmex-5::Cas9 expression vector |
| 188883 | pMLS546 | Integrating Pmex-5::Cas9 expression vector |
| 188884 | pMLS597 | dpy-10 sgRNA expression vector |
| 188885 | pMLS713 | Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker |
| 188886 | pMLS714 | MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119 |
| 188887 | pMLS715 | Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm |
| 188888 | pMLS716 | Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre |
| 188889 | pMLS791 | Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP |
| 188890 | pMLS485 | Worm Cas9 expression vector, Peft-3 promoter |
| 188891 | pMLS1272 | Ric-4 sgRNA expression vector |
| 188892 | pMLS640 | ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker |
| 188893 | pMLS621 | ttTi5605 MosSci targeting vector with floxed Cbr-unc-119 marker |
| 188894 | pMLS719 | miniMos Arm sgRNA expression vector |