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Cytosine base editing systems with minimized off-target effect and molecular size.

Li A, Mitsunobu H, Yoshioka S, Suzuki T, Kondo A, Nishida K
Nat Commun. 2022 Aug 8;13(1):4531. doi: 10.1038/s41467-022-32157-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188646Yeast_Target-AID3SExpresses Target-AID3S in yeasts
188647Yeast_Target-AID2SExpressing Target-AID2S in yeasts
188648Mammalian_SpCas9_Target-AID2SExpresses SpCas9_Target-AID2S in mammalian cells
188649Mammalian_SpCas9_Target-AID3SExpresses SpCas9_Target-AID3S in mammalian cells
188651Mammalian_SaAID3SExpresses SaAID3S and gRNA in mammalian cells
188652Mammalian_SaAID2SExpresses SaAID2S and gRNA in mammalian cells
188653Mammalian_SaAIDExpresses SaAID in mammalian cells
188654AAV_A2S-8AAV vector expressing SaAID-2S and gRNA

Antibodies from Article