Structure-function analysis of microRNA 3'-end trimming by Nibbler.
Xie W, Sowemimo I, Hayashi R, Wang J, Burkard TR, Brennecke J, Ameres SL, Patel DJ
Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30370-30379. doi: 10.1073/pnas.2018156117. Epub 2020 Nov 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188584 | pAFMW-Nbr | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188585 | pAFMW-Nbr-CM | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188586 | pAFMW-Nbr[N1-383] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188587 | pAFMW-Nbr[C399-625] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188588 | pAFMW-Nbr[C399-625 CM] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188589 | pAFMW-Nbr[C399-625]-Gwpep(Mm) | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188590 | pAFMW-Nbr[C399-625]-Gwpep(Dm) | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188591 | pAFMW-Gwpep(Mm)-Nbr[C399-625] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188592 | pAFMW-Gwpep(Dm)-Nbr[C399-625] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188593 | pAFMW-Nbr[K187A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188594 | pAFMW-Nbr[R272A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188595 | pAFMW-Nbr[K284A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188596 | pAFMW-Nbr[R272A,K276A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188597 | pAFMW-Nbr[K276A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188598 | pAFMW-Nbr[R280A,K284A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188599 | pAFMW-Nbr[R272A,K276A, R280A,K284A] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |
| 188600 | pAFMW-Nbr[K216E] | Flag-tagged Nibbler variants expression in Drosophila S2 cells |