Human pluripotent stem cell modeling of alveolar type 2 cell dysfunction caused by ABCA3 mutations.
Sun YL, Hennessey EE, Heins H, Yang P, Villacorta-Martin C, Kwan J, Gopalan K, James M, Emili A, Cole FS, Wambach JA, Kotton DN
J Clin Invest. 2024 Jan 16;134(2):e164274. doi: 10.1172/JCI164274.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188545 | pX330-U6-E690_guide-CBh-hSpCas9 | Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690 |
| 188546 | pX330-U6-W308_guide-CBh-hSpCas9 | Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308 |
| 188547 | pX330-U6-L101_guide-CBh-hSpCas9 | Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101 |
| 188548 | pHAGE-EF1aL-WT_ABCA3-GFP | Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein |
| 188549 | pHAGE-EF1aL-E690Kmut_ABCA3-GFP | Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein |
| 188550 | pHAGE-EF1aL-W308Rmut_ABCA3-GFP | Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein |