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Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters.

Sun YL, Hurley K, Villacorta-Martin C, Huang J, Hinds A, Gopalan K, Caballero IS, Russo SJ, Kitzmiller JA, Whitsett JA, Beers MF, Kotton DN
Am J Respir Cell Mol Biol. 2021 Oct;65(4):442-460. doi: 10.1165/rcmb.2020-0259OC. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188540phABCA3-eGFP_CRISPR_DonorHomologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus
188541pSPCas9(BB)-2A-GFP_ABCA3GFP_targetingPlasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Antibodies from Article