Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells.
Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188489 | SpCas9 | Expresses FLAG tagged SpCas9 |
| 188490 | HiFi Cas9 | Expresses FLAG tagged HiFi Cas9 |
| 188491 | SpRY Cas9 | Expresses FLAG tagged SpRY Cas9 |
| 188492 | AsCas12a | Expresses FLAG tagged AsCas12a |
| 188493 | LbCas12a | Expresses FLAG tagged LbCas12a |
| 188494 | SaCas9 | Expresses FLAG tagged SaCas9 |
| 188495 | DpbCas12e | Expresses FLAG tagged DpbCas12e |
| 188496 | PlmCas12e | Expresses FLAG tagged PlmCas12e |
| 188497 | Cas12j3 | Expresses FLAG tagged Cas12j3 |
| 188498 | Cas12j2 | Expresses FLAG tagged Cas12j2 |
| 188499 | SpdCas9 KRAB | Expresses FLAG tagged SpdCas9 KRAB |
| 188500 | HiFi dCas9 KRAB | Expresses FLAG tagged HiFi dCas9 KRAB |
| 188501 | SpRY dCas9 KRAB | Expresses FLAG tagged SpRY dCas9 KRAB |
| 188502 | AsdCas12a KRAB | Expresses FLAG tagged AsdCas12a KRAB |
| 188503 | LbdCas12a KRAB | Expresses FLAG tagged LbdCas12a KRAB |
| 188504 | SadCas9 KRAB | Expresses FLAG tagged SadCas9 KRAB |
| 188505 | DpbdCas12e KRAB | Expresses FLAG tagged DpbdCas12e KRAB |
| 188506 | PlmdCas12e KRAB | Expresses FLAG tagged PlmdCas12e KRAB |
| 188507 | dCas12j3 KRAB | Expresses FLAG tagged dCas12j3 KRAB |
| 188508 | dCas12j2 KRAB | Expresses FLAG tagged dCas12j2 KRAB |
| 188509 | SpdCas9 VPR | Expresses FLAG tagged SpdCas9 VPR |
| 188510 | HiFi dCas9 VPR | Expresses FLAG tagged HiFi dCas9 VPR |
| 188511 | SpRY dCas9 VPR | Expresses FLAG tagged SpRY dCas9 VPR |
| 188512 | AsdCas12a VPR | Expresses FLAG tagged AsdCas12a VPR |
| 188513 | LbdCas12a VPR | Expresses FLAG tagged LbdCas12a VPR |
| 188514 | SadCas9 VPR | Expresses FLAG tagged SadCas9 VPR |
| 188515 | DpbdCas12e VPR | Expresses FLAG tagged DpbdCas12e VPR |
| 188516 | PlmdCas12e VPR | Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta) |
| 188517 | dCas12j3 VPR | Expresses FLAG tagged dCas12j3 VPR |
| 188518 | dCas12j2 VPR | Expresses FLAG tagged dCas12j2 VPR |
| 188519 | miniCMV eGFP hPGK BSD | Backbone for CRISPR activation (CRISPRa) isogenic PAM testing |
| 188520 | EFS eGFP hPGK BSD | Backbone for CRISPR interference (CRISPRi) isogenic PAM testing |