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An optogenetic toolkit for light-inducible antibiotic resistance.

Sheets MB, Tague N, Dunlop MJ
Nat Commun. 2023 Feb 23;14(1):1034. doi: 10.1038/s41467-023-36670-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188474MG1655-OptoCre-bla-P-RCre-inducible activation of bla for ampicillin/carbenicillin resistance.
188475MG1655-OptoCre-knt-P-RCre-inducible activation of knt for kanamycin resistance.
188476MG1655-OptoCre-knt-P*-RCre-inducible activation of knt for kanamycin resistance.
188477MG1655-OptoCre-knt-P**-RCre-inducible activation of knt for kanamycin resistance.
188478MG1655-OptoCre-knt-P-R*Cre-inducible activation of knt for kanamycin resistance.
188479MG1655-OptoCre-cat-P-RCre-inducible activation of catfor chloramphenicol resistance.
188480MG1655-OptoCre-cat-T172A-P-RCre-inducible activation of catT172Afor chloramphenicol resistance.
188481MG1655-OptoCre-tetA-Ptet-RtetCre-inducible activation of tetA for tetracycline resistance.
189849p15A-OptoCre-knt-P-RCre-inducible activation of knt for kanamycin resistance.
189850p15A-OptoCre-knt-P*-RCre-inducible activation of knt for kanamycin resistance.
189851p15A-OptoCre-knt-P**-RCre-inducible activation of knt for kanamycin resistance.
189852p15A-OptoCre-knt-P-R*Cre-inducible activation of knt for kanamycin resistance.
189853p15A-OptoCre-cat-P-RCre-inducible activation of cat for chloramphenicol resistance.
189854p15A-OptoCre-cat-T172A-P-RCre-inducible activation of cat-T172A for chloramphenicol resistance.
189855p15A-OptoCre-tetA-Ptet-RtetCre-inducible activation of tetA for tetracycline resistance.

Antibodies from Article