MicroID2: A Novel Biotin Ligase Enables Rapid Proximity Dependent Proteomics.
Johnson BS, Chafin L, Farkas D, Adair J, Elhance A, Farkas L, Bednash JS, Londino JD
Mol Cell Proteomics. 2022 Jun 7:100256. doi: 10.1016/j.mcpro.2022.100256.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188521 | pSBbi_RP_MicroID2-HA | Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells |
| 188522 | pSBbi_RP_lbMicroID2-HA | Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells |
| 188523 | pSBbi_RP_3XNLS-MicroID2-FLAG | Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells |
| 188524 | pSBbi_RP_2XMTS-MicroID2-FLAG | Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells |
| 188525 | pSBbi_RP_MicroID2-FLAG-NES | Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells |