Machine-learning-optimized Cas12a barcoding enables the recovery of single-cell lineages and transcriptional profiles.

Hughes NW, Qu Y, Zhang J, Tang W, Pierce J, Wang C, Agrawal A, Morri M, Neff N, Winslow MM, Wang M, Cong L
Mol Cell. 2022 Jun 22. pii: S1097-2765(22)00537-8. doi: 10.1016/j.molcel.2022.06.001. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
188470	pN006	Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)
188471	pN251	Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)
188472	pN256	scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP