Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-beta signaling.

Mishra T, Bhardwaj V, Ahuja N, Gadgil P, Ramdas P, Shukla S, Chande A
Mol Ther Nucleic Acids. 2022 Mar 8;28:202-218. doi: 10.1016/j.omtn.2022.03.003. eCollection 2022 Jun 14. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
188449	pScalps eSpCas9_Zsgreen gRNA scaffold	Expression of eSpCas9 tagged with Zsgreen
188450	pTZ tRNA_gRNA empty scaffold	Expression of gRNAs using a human tRNA promoter
188451	pmGFP	Expression of a modified GFP (mGFP) with short half-life