Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer.

Lu K, Wazawa T, Sakamoto J, Vu CQ, Nakano M, Kamei Y, Nagai T
Nano Lett. 2022 Jul 27;22(14):5698-5707. doi: 10.1021/acs.nanolett.2c00608. Epub 2022 Jul 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
188446	B-gTEMP/pRSET-b	B-gTEMP for bacterial expression
188447	B-gTEMP/pcDNA3	B-gTEMP for mammalian expression
199806	mtsCOXVIIIx2-B-gTEMP/pcDNA3	Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence