Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression.
Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188396 | pDR111_Bgal NLS | Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase. |
| 188397 | pDR111_Bgal no NLS | Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase. |
| 188398 | pDR111_mannose_Bgal NLS | Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase. |
| 188399 | pDR111_mannose_Bgal no NLS | Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase. |
| 188400 | pDR111_mannose_Stat1Klf6 | Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors. |
| 188401 | pDR111_mannose_Klf4Gata-3 | Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors. |