Modular design of synthetic receptors for programmed gene regulation in cell therapies.
Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, Israni DV, Liu B, Khalil AS, Roybal KT
Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 10.1016/j.cell.2022.03.023.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188336 | pHR_PGK_SNIPR_hsNotch1 LNR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188338 | pHR_PGK_SNIPR_hsNotch3 LNR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor |
| 188340 | pHR_PGK_SNIPR_synRobo | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Robo1-TMD, a Robo1-JMD, and a Gal4VP64 transcriptional factor |
| 188341 | pHR_PGK_SNIPR_synRobo/Notch | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188342 | pHR_PGK_SNIPR_FnIII-Notch | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188343 | pHR_PGK_SNIPR_(GGS)3_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)3, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188344 | pHR_PGK_SNIPR_(GGS)6_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188345 | pHR_PGK_SNIPR_(GGS)9_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)9, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188346 | pHR_PGK_SNIPR_(GGS)12_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)12, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188347 | pHR_PGK_SNIPR_(GGS)15_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)15, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188348 | pHR_PGK_SNIPR_(GGS)18_SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)18, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188349 | pHR_PGK_SNIPR_ADAM17 site | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188350 | pHR_PGK_SNIPR_FAP site | Lentiviral vector - constitutive expression of an myctag SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188351 | pHR_PGK_SNIPR_FAP site with scFv | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188352 | pHR_PGK_SNIPR_3xFLAG-tag tether | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a 3X-FLAG-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188353 | pHR_PGK_SNIPR_Notch1 del-NRR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188354 | pHR_PGK_SNIPR_Notch2 del-NRR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch2-del-NRR-ECD, a Notch2-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor |
| 188355 | pHR_PGK_SNIPR_Notch3 del-NRR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-del-NRR-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor |
| 188357 | pHR_PGK_SNIPR_CD8alpha SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188358 | pHR_PGK_SNIPR_CD28 SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188359 | pHR_PGK_SNIPR_IgG4 SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a IgG4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188360 | pHR_PGK_SNIPR_OX40 SNIPR | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a OX40-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188362 | pHR_PGK_SNIPR_CD8alpha variant 2 | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188363 | pHR_PGK_SNIPR_CD8alpha variant 3 | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188364 | pHR_PGK_SNIPR_CD8alpha variant 4 | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188365 | pHR_PGK_SNIPR_CD8alpha variant 2, G318A | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188366 | pHR_PGK_SNIPR_CD8alpha variant 2, V319A | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188374 | pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor |
| 188375 | pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD) | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor |
| 188376 | pHR_PGK_ALPPL2 synNotch | Lentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factor |
| 188377 | pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR | Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor |
| 188379 | pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD) | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor |
| 188380 | pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17) | Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor |
| 188383 | pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6) | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor |
| 188384 | pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A) | Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor |
| 188385 | pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv) | Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor |
| 188386 | pHR_Gal4UAS_tBFP_PGK_mCitrine | Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine; |
| 188387 | pHR_HNF1A-RE_tBFP_PGK_mCitrine | Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine; |
| 188388 | pHR_Pax6-RE_tBFP_PGK_mCitrine | Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine |